Plant Circular RNAs

Plant Circular RNAs

Methods and Protocols

Vaschetto, Luis Maria

Springer-Verlag New York Inc.

07/2022

196

Mole

Inglês

9781071616475

15 a 20 dias

404

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Identification and Characterization of Plant Circular RNAs.- Experimental Strategies for Studying the Function of Plant circRNAs.- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization.- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development.- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing.- Identification and Functional Characterization of Viroid Circular RNAs.- Circular RNA Databases.- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs.- Computational Analysis of Transposable Elements and circRNAs in Plants.- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database.- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.
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Quantitative real-time PCR (qRT-PCR);RNA immunoprecipitation;circular RNAs (circRNAs);sequencing data;comparative genomics