DNA Modification Detection Methods

Part 1. Detection of 5mC, 5hmC, 5fC, and 5caC.- Chapter 1. Quantitative assessment of the oxidation products of 5-methylcytosine in DNA by liquid chromatography-tandem mass spectrometry.- Chapter 2. Determination of cytosine modifications in DNA by chemical labeling-mass spectrometry analysis.- Chapter 3. Analysis of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by capillary electrophoresis-mass spectrometry.- Chapter 4. Simple quantification of epigenetic DNA modifications and DNA damage on multi-well slides.- Chapter 5. Label-free and immobilization-free electrochemical magnetobiosensor for sensitive detection of 5-hydroxymethylcytosine in genomic DNA.- Chapter 6. Electrochemical assay for continuous monitoring of dynamic DNA methylation process.- Chapter 7. Electrogenerated chemiluminescence method for determination of 5-hydroxymethylcytosine in DNA.- Chapter 8. Quantification of site-specific 5-formylcytosine by integrating peptide nucleic acid-clamped ligation with loop-mediated isothermal amplification.- Chapter 9. Global DNA methylation analysis using methylcytosine dioxygenase.- Part 2. Detection of 6mA.- Chapter 10. Metabolically generated stable isotope for identification of DNA N6-methyladenine origin in cultured mammalian cells.- Chapter 11. Determination of N6-methyladenine in DNA of mammals and plants by Dpn I digestion combined with size-exclusion ultrafiltration and mass spectrometry analysis.- Part 3. Detection of 5hmU and 5fU.- Chapter 12. Isotope-dilution liquid chromatography-tandem mass spectrometry for detection of 5-hydroxymethyluracil and 5-formyluracil in DNA.- Chapter 13. Detection of 5 Formylcytosine and 5 Formyluracil based on Photo-assisted Domino Reaction.- Chapter 14. Detection of 5-formyluracil and 5-formylcytosine in DNA by fluorescence labelling.- Part 4. Detection of Base J and 8-oxo-7, 8-dihydroguanine.- Chapter 15. Mass spectrometry-based quantification of ?-D-glucosyl-5-hydroxymethyluracil in genomic DNA.- Chapter16. Determination of 8-oxo-7,8-dihydroguanine in DNA at single-base resolution by polymerase-mediated differential coding.

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• Biologia, ciências da vida
• Genética (não médica)
• Biotecnologia
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DNA modification;Detection;Mapping;Mass spectrometry;DNA Sequencing;gene expression