Complete Guide to Gene Cloning: From Basic to Advanced

1.The Recombinant DNA technology era.- 2.Vectors: Guide to gene delivery vehicles.- 3.Gene isolation methods: beginner's guide.- 4.Restriction Enzymes-Tools for DNA cloning.- 5.Overview of Gene cloning strategies.- 6.Fundamental techniques of recombinant DNA transfer.- 7.Selection, screening and analysis of recombinant clones.- 8.Recent trends and advances.- 9.Applications of Recombinant DNA technology.- 10.Ethical and safety concerns of recombinant DNA Technology.

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• Equipamentos científicos, experiências e técnicas
• DNA e Genoma
• Biologia molecular
• Genética (não médica)
• Biologia, ciências da vida
• Biotecnologia

Recombinant DNA Technology;Vectors;Restriction enzymes;Inserts;Ligation;DNA Transfer;Recombinants;Medical ethics;PCR;Western blotting;Southern blotting;Sequencing;Bacteriophage;Plasmid;Methylation;cDNA library;Golden gate;CRISPR;Gibson;Dicistronic cloning