P2X7 Receptor
portes grátis
P2X7 Receptor
Methods and Protocols
Nicke, Annette
Springer-Verlag New York Inc.
07/2022
394
Dura
Inglês
9781071623831
15 a 20 dias
961
Descrição não disponível.
How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating.- Agonists, Antagonists, and Modulators of P2X7 Receptors.- Modulation of Cell Energy Metabolism by the P2X7 Receptor.- Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE.- Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4).- Development of Antibody and Nanobody Tools for P2X7.- Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors.- Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations.- Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes.- A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors.- Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents.- Using SymmetricalOrganic Cation Solutions to Study P2X7 Ion Permeation.- Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors.- Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry.- Characterization of P2X7 Receptors in Human Blood Cells.- Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube.- Administration of P2X7 Receptor Blockers in Oncological Experimental Models.- Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease.- Analysis of P2X7-Induced Neuronal Branching.- Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique.- Analyzing the Role of the P2X7 Receptor in Epilepsy.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
Receptors;Ion channels;Drug targets;Channel activation;Antibody and nanobody tools;Down-stream signaling
How Structural Biology Has Directly Impacted Our Understanding of P2X Receptor Function and Gating.- Agonists, Antagonists, and Modulators of P2X7 Receptors.- Modulation of Cell Energy Metabolism by the P2X7 Receptor.- Fully Flexible Ligand Docking for the P2X7 Receptor Using ROSIE.- Preparation of the Murine Anti-Human P2X7 Receptor Monoclonal Antibody (Clone L4).- Development of Antibody and Nanobody Tools for P2X7.- Enhanced Transduction of P2X7-Expressing Cells with Recombinant rAAV Vectors.- Immunofluorescence Staining of P2X7 Receptors in Whole-Mount Myenteric Plexus Preparations.- Established Protocols for cRNA Expression and Voltage-Clamp Characterization of the P2X7 Receptor in Xenopus laevis Oocytes.- A Simplified Protocol to Incorporate the Fluorescent Unnatural Amino Acid ANAP into Xenopus laevis Oocyte-Expressed P2X7 Receptors.- Using Whole-Cell Electrophysiology and Patch Clamp Photometry to Characterize P2X7 Receptor Currents.- Using SymmetricalOrganic Cation Solutions to Study P2X7 Ion Permeation.- Methods for Studying Cholesterol-Dependent Regulation of P2X7 Receptors.- Visualizing P2X7-Dependent Inflammasome Formation in Human Monocytes by Fluorescence Microscopy and Flow Cytometry.- Characterization of P2X7 Receptors in Human Blood Cells.- Real-Time Flow Cytometry as a Tool to Monitor Cellular Consequences of P2X7 Activation in Multiple Cell Populations Mixed in a Single FACS Tube.- Administration of P2X7 Receptor Blockers in Oncological Experimental Models.- Humanized Mouse Model to Study the P2X7 Receptor in Graft-Versus-Host Disease.- Analysis of P2X7-Induced Neuronal Branching.- Studying the Role of P2X7 Receptor in Axonal Growth Using In Utero Electroporation Technique.- Analyzing the Role of the P2X7 Receptor in Epilepsy.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
