Textbook on Cloning, Expression and Purification of Recombinant Proteins

A Brief Introduction to Recombinant DNA Technology.- Cloning and Gene Manipulation.- Selection of Cloning and Expression Plasmid Vectors.- Transformation and Protein Expression.- Introduction to Recombinant Protein Purification.- Protein Purification by Affinity Chromatography.- Protein Purification by Ion Exchange Chromatography.- Gel Filtration Chromatography.- Protein Purification by Reversed Phase Chromatography and Hydrophobic Interaction Chromatography.- Purification of Difficult Proteins.- Protein Quantitation and Detection.

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• Biofísica
• Ciências da vida: temas gerais
• Biotecnologia
• Genética médica

Recombinant DNA technology;Gene cloning;Expression vectors;Protein purification;Protein quantitation