Principles of Light Microscopy: From Basic to Advanced

Chapter 1. The physical principles of light propagation and light-matter interactions.- Chapter 2. Design, alignment and usage of infinity-corrected microscope.- Chapter 3. Epifluorescence Microscopy.- Chapter 4. Basic digital image design, processing, analysis, management, and presentation.- Chapter 5. Confocal Microscopy.- Chapter 6. Live-cell Imaging: A Balancing Act Between Speed, Sensitivity and Resolution.- Chapter 7. Structured Illumination Microscopy.- Chapter 8. Stimulated Emission Depletion Microscopy.- Chapter 9. Two-Photon Imaging.- Chapter 10. Modern microscopy image analysis: quantifying colocalization on a mobile device.- Chapter 11. Spinning Disk Microscopy.- Chapter 12. Light Sheet Fluorescence Microscopy.- Chapter 13. Localization microscopy: a review of the progress in methods and applications.

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• Biologia, ciências da vida
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• Microscopia
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microscopy;fluorescence;confocal;super-resultion;STED;SIM;STORM;PALM;imaging;histology;introduction to microscopy;popular imaging systems;imaging facilities;laboratory techniques;methods and protocols