Methods for Fragments Screening Using Surface Plasmon Resonance
portes grátis
Methods for Fragments Screening Using Surface Plasmon Resonance
Zaheer, Sameer Mahmood; Gosu, Ramachandraiah
Springer Verlag, Singapore
09/2021
113
Dura
Inglês
9789811615351
15 a 20 dias
371
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Chapter 1. Introduction to Surface Plasmon Resonance.- Chapter 2. Principle of Surface Plasmon Resonance (OneStep).- Chapter 3. Preparation of protein with Histidine Tag for Amine coupling.- Chapter 4. Preparation of protein with Avidin Tag for Biotin-based Capture.- Chapter 5. Protein stability using Thermal Shift Assay (TSA) - pH Tolerance.- Chapter 6. Protein stability using Thermal Shift Assay (TSA) - DMSO Tolerance.- Chapter 7. Selection and Identification of Fragment library.- Chapter 8. Preparation of Sensor.- Chapter 9. Optimization and validation of Amine coupling of Ligand.- Chapter 10. Optimization and Validation of Strepavidin/Biotin-based capture of Ligand.- Chapter 11. Preparation of Fragments for Screening in SPR.- Chapter 12. Screening of Fragments in SPR.- Chapter 13. Identification of Fragment Hits.- Chapter 14. Data Analysis and Confirmation of Hits.
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Surface plasmon resonance;Protein stability;Fragment library;Thermal shift assay;Amine coupling
Chapter 1. Introduction to Surface Plasmon Resonance.- Chapter 2. Principle of Surface Plasmon Resonance (OneStep).- Chapter 3. Preparation of protein with Histidine Tag for Amine coupling.- Chapter 4. Preparation of protein with Avidin Tag for Biotin-based Capture.- Chapter 5. Protein stability using Thermal Shift Assay (TSA) - pH Tolerance.- Chapter 6. Protein stability using Thermal Shift Assay (TSA) - DMSO Tolerance.- Chapter 7. Selection and Identification of Fragment library.- Chapter 8. Preparation of Sensor.- Chapter 9. Optimization and validation of Amine coupling of Ligand.- Chapter 10. Optimization and Validation of Strepavidin/Biotin-based capture of Ligand.- Chapter 11. Preparation of Fragments for Screening in SPR.- Chapter 12. Screening of Fragments in SPR.- Chapter 13. Identification of Fragment Hits.- Chapter 14. Data Analysis and Confirmation of Hits.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
