Marburg Virus

Marburg Virus

Methods and Protocols

Banadyga, Logan; Marzi, Andrea

Springer-Verlag New York Inc.

01/2025

455

Dura

9781071642559

Pré-lançamento - envio 15 a 20 dias após a sua edição

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Mismatch of Supply and Demand: Marburg Virus Disease Outbreaks Need Countermeasures but Will Not Provide Opportunity for Clinical Trials.- Marburg Virus Medical Countermeasures.- Isolation and Propagation of Marburg viruses.- Validation of Marburg Virus Inactivation by AVL Buffer.- Generation, Recovery, and Propagation of a Recombinant Vesicular Stomatitis Virus Expressing the Marburg Virus Glycoprotein.- Visualizing the Internalization of Marburg Virus-Like Particles into Living Cells.- Proximity Proteomics to Profile Ebola Virus Protein Interactome in its Functional Context.- Monoclonal Antibody Production Against Filoviruses from Immunized Mice.- Marburg Virus Minigenome Assays.- Transcription- and Replication-Competent Virus-Like Particle Systems for Marburg Virus.- Generation of Biologically Contained Marburg Virus.- Recovery of Recombinant Marburg Virus by Reverse Genetics.- In Vivo Investigation of Filovirus Glycoprotein-Mediated Infection in a BSL2 Setting.- Evaluating Countermeasures against Marburg Virus Using a Mouse Model.- Filovirus Infection in Humanized Mouse Models.- A Hamster Model for the Evaluation of Marburg Virus Countermeasures.- Evaluation of Marburg Virus Medical Countermeasures in Guinea Pigs.- Experimental Infection of Egyptian Rousette Bats (Rousettus aegyptiacus) with Marburg Virus.- Evaluation of Vaccines and Therapeutics Against Ebolaviruses in the Domestic Ferret.- Evaluation of Vaccines and Therapeutics against Marburg Virus in Nonhuman Primate Models.- Marburg Virus Aerosol Infection in Animal Models.- Gross and Histopathologic Evaluation of Tissues from Marburg Virus Infected Nonhuman Primates.- Detection of Serum Antibodies Targeting the Marburg Virus Glycoprotein using a Multiplex Immunoassay Platform.- Quantification of Neutralizing Antibodies in Serum using VSV-MARV-GFP.- Cellular Phenotyping of Peripheral Blood Mononuclear Cells from Marburg Virus-Infected Animals.- Transcriptional Profiling of Filovirus-Infected Nonhuman Primate Samples.- Droplet-Based Single Cell 3' mRNA Sequencing of Marburg Virus Infected Samples.- Quantification of Filovirus Soluble Glycoprotein using a Sandwich ELISA.- Immunochromatographic Lateral Flow Assays to Detect Filovirus Nucleoproteins.- Amplification of Filovirus Genomes from Clinical Samples for Next Generation Sequencing.- Virus Stability on Surfaces, in Bodily Fluids, in Wastewater, and Different Environmental Conditions.
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Filoviruses;Recombinant engineering;Virus culture;Sample analysis;Animal modeling