Transposable Elements

Transposable Elements

Methods and Protocols

de Mendoza Soler, Alexandre; Branco, Miguel R.

Springer-Verlag New York Inc.

12/2022

382

Dura

Inglês

9781071628829

15 a 20 dias

940

Descrição não disponível.
An overview of best practices for transposable element identification, classification and annotation in eukaryotic genomes.- Assembly-free annotation and quantification of transposable elements with dnaPipeTE.- Best practice for the identification of horizontally transferred transposons.- Genotyping of transposable elements insertions segregating in the human populations using short-read re-alignments.- A Pangenome approach to detect and genotype TE insertion polymorphisms.0 Experimental validation of transposable element insertions using the Polymerase Chain Reaction (PCR).- Quantification of LINE-1 RNA expression from bulk RNA-seq using L1EM.- Genome-wide profiling of L1 DNA methylation by bs-ATLAS-seq.- Nanopore epigenomic analysis of transposable element DNA modifications.- Targeted resequencing and methylation analysis of L1 elements by nanopore sequencing.- Inferring protein-DNA binding profiles at interspersed repeats using HiChIP and PatChER.- Affinity-based Interactome Analysis of Endogenous LINE-1 Macromolecules.- LINE-1 retrotransposition assays in embryonic stem cells.- Detecting somatic transposable element insertions in Drosophila tissues.- Precise and scarless insertion of transposable elements by Cas9-mediated genome engineering.- Epigenetic editing of transposable and repetitive elements.- Using CRISPR to investigate the regulatory activity of transposable elements.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
KZNFs;polymorphic transposons;Nanopore;ChIP-seq;CRISPR-Cas9