SILAC

SILAC

Methods and Protocols

Luque-Garcia, Jose L.

Springer-Verlag New York Inc.

11/2022

286

Dura

Inglês

9781071628621

15 a 20 dias

765

Descrição não disponível.
Dynamic SILAC to Determine Protein Turnover in Neurons and Glia.- SILAC-Based Proteomic Analysis of Meiosis in the Fission Yeast Schizosaccharomyces pombe.- Identification of Protein Interaction Partners in Bacteria Using Affinity Purification and SILAC Quantitative Proteomics.- pSILAC-Based Determination of Cellular Protein Sorting into Extracellular Vesicles.- Quantification of Protein Palmitoylation by Cysteine-SILAC.- Comparative SUMO Proteome Analysis Using Stable Isotopic Labeling by Amino Acids (SILAC).- A Super-SILAC Approach for Profiling Histone Post-Translational Modifications.- SILAC-Based Quantitative Phosphoproteomics in Yeast.- Phosphotyrosine Profiling Using SILAC.- Chemoproteomic Profiling of Geranyl Pyrophosphate-Binding Proteins.- Quantitative Analysis of the Endoplasmic Reticulum-Associated Proteins Using ER-Localizable Reactive Molecules.- Workflow for Quantitative Proteomic Analysis of Intestinal Organoids Using SILAC.- Stable Isotope Labeling by Amino Acids and Bioorthogonal Noncanonical Amino Acid Tagging in Cultured Primary Neurons.- Heavy Methyl SILAC Metabolic Labeling of Human Cell Lines for High-Confidence Identification of R/K-Methylated Peptides by High-Resolution Mass Spectrometry.- SILAC-Based Quantitative Proteomic Analysis of Drosophila Embryos.- Super-SILAC Quantitative Proteome Profiling of Zebrafish Larvae.- Use of Nuclear and Chromatin Enrichment Procedures for Quantitation of Yeast DNA Replication Proteins Using SILAC.- Combination of Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) and Substrate Trapping for the Detection of Transient Protein Interactions.- Profiling of Secreted Proteins in Serum-Containing Media Using BONCAT and Pulsed SILAC.- Using SILAC to Develop Quantitative Data Independent Acquisition (DIA) Proteomics Methods.- SILAC-IodoTMT for Assessment of the Cellular Proteome and Its Redox Status.- Targeted Absolute Protein Quantification Using SILAC Internal Standard and Full-LengthProtein Calibrators (TAQSI).
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Quantitative proteomics;Stable isotope labeling;Bioinformatics;Functional proteomics;Mass spectrometry