Protocols for the Diagnosis of Pig Viral Diseases

Protocols for the Diagnosis of Pig Viral Diseases

Yadav, Ajay Kumar; Deb, Rajib; Rajkhowa, Swaraj; Malik, Yashpal Singh

Springer-Verlag New York Inc.

05/2022

381

Dura

Inglês

9781071620427

15 a 20 dias

947

Descrição não disponível.
Requirements and Preparedness for Attending a Viral Disease Outbreak in Pig Farms.- Collection of Samples, Their Preservation, and Transportation.- Methods for Quantification of Viruses.- Protocols for Isolation of Genetic Materials from RNA Viruses.- Multiplex PCR for Diagnosis of Porcine Diseases.- Protocols for Isolation of Plasmid DNA.- Recombinant Antigen-Based Diagnostic Assays of Pig Viral Diseases.- RNA-PAGE-Based Diagnosis of Viral Diseases.- Peptide Nucleic Acid (PNA): A Diagnostic Molecule for Infectious Diseases.- Nucleic Acid Sequence-Based Amplification (NASBA) Methods and CRISPR/Cas13 System to Detect Pig Viral Diseases.- Aptamers as Diagnostic Markers for Viral Infections of Veterinary Importance.- Antibody-Based Sensors for Pathogen Detection.- Lateral Flow Assay for Diagnosis of Pig Viral Diseases.- Droplet Digital PCR-Based Diagnosis for Porcine Viral Diseases.- Protocols for Immunofluorescence Techniques.- Polymerase Spiral Reaction (PSR) for the Diagnosis of Porcine Viral Diseases.- Recombinase Polymerase Amplification-Based Diagnostics of Porcine Viral Diseases.- Cell Culture System for Porcine Virus Isolation and Propagation.- An Overview of Mouse Monoclonal Antibody Production.- Nucleic Acid Hybridization Techniques for Viral Disease Diagnosis: A Detailed Perspective.- Ligase Detection Reaction-Fluorescent Microsphere Assay.- ELISA as a Diagnostic Weapon.- SDS-PAGE and Western Blotting: Basic Principles and Protocol.- Immune Assays as Diagnostic for Pig Viral Diseases.- Production of Virus-Like Particles Using the Baculovirus Expression System and Their Application in Vaccines and Viral Disease Diagnosis.- Good Laboratory Practices and Biosafety Containments in a Virology Laboratory.
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Immune assay-based diagnostics;Porcine pathogens;Zoonotic diseases;Swine disease;Domestic and wild pigs;Piggery;Antigens and antibodies