Optical Tweezers
portes grátis
Optical Tweezers
Methods and Protocols
Gennerich, Arne
Springer-Verlag New York Inc.
09/2022
757
Dura
Inglês
9781071622285
15 a 20 dias
1630
Descrição não disponível.
The Invention of the Laser.- Art Ashkin and the Origins of Optical Trapping and Particle Manipulation.- Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes.- Angular Optical Trapping to Directly Measure DNA Torsional Mechanics.- Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System.- One-Dimensional STED Microscopy in Optical Tweezers.- Temperature Quantification and Temperature Control in Optical Tweezers.- High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy.- Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers.- Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay.- Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers.- Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding.- CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy.- Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation.- Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone.- Tethering Complex Proteins and Protein Complexes for Optical Tweezers Experiments.- Single-Molecule Manipulation Study of Chaperoned SNARE Folding and Assembly with Optical Tweezers.- Using Optical Tweezers to Monitor Allosteric Signals through Changes in Folding Energy Landscapes.- High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors.- Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors.- Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A.- Ultra-Fast Force-Clamp Spectroscopy of Microtubule-Binding Proteins.- Catching the Conformational Wave: Measuring the Working Strokesof Protofilaments as They Curl Outward from Disassembling Microtubule Tips.- Mechanics of Single Vimentin Intermediate Filaments under Load.- Quantifying the Interaction Strength between Biopolymers.- Measuring ?? T Cell Receptor-Mediated Mechanosensing Using Optical Tweezers Combined with Fluorescence Imaging.
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Single-molecule experiments;DNA-protein interactions;Protein folding;Motor proteins;Microtubules;Mechanosensing;Optical traps
The Invention of the Laser.- Art Ashkin and the Origins of Optical Trapping and Particle Manipulation.- Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes.- Angular Optical Trapping to Directly Measure DNA Torsional Mechanics.- Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System.- One-Dimensional STED Microscopy in Optical Tweezers.- Temperature Quantification and Temperature Control in Optical Tweezers.- High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy.- Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers.- Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay.- Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers.- Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding.- CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy.- Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation.- Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone.- Tethering Complex Proteins and Protein Complexes for Optical Tweezers Experiments.- Single-Molecule Manipulation Study of Chaperoned SNARE Folding and Assembly with Optical Tweezers.- Using Optical Tweezers to Monitor Allosteric Signals through Changes in Folding Energy Landscapes.- High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors.- Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors.- Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A.- Ultra-Fast Force-Clamp Spectroscopy of Microtubule-Binding Proteins.- Catching the Conformational Wave: Measuring the Working Strokesof Protofilaments as They Curl Outward from Disassembling Microtubule Tips.- Mechanics of Single Vimentin Intermediate Filaments under Load.- Quantifying the Interaction Strength between Biopolymers.- Measuring ?? T Cell Receptor-Mediated Mechanosensing Using Optical Tweezers Combined with Fluorescence Imaging.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
