Lipid Rafts

Lipid Rafts

Methods and Protocols

Bieberich, Erhard

Springer-Verlag New York Inc.

08/2021

350

Mole

Inglês

9781071608166

15 a 20 dias

692

Descrição não disponível.
Isolation and Analysis of Lipid Rafts from Neural Cells and Tissues.- A Detergent-Free Method for Preparation of Lipid Rafts for the Shotgun Lipidomics Study.- Biochemical Analysis of Lipid Rafts to Study Pathogenic Mechanisms of Neural Diseases.- Amyloid- ? Interactions with Lipid Rafts in Biomimetic Systems: A Review of Laboratory Methods.- Extracellular Vesicles Containing Ceramide-Rich Platforms: "Mobile Raft" Isolation and Analysis.- Isolation of Lipid Rafts (Detergent-Resistant Microdomains) and Comparison to Extracellular Vesicles (Exosomes).- Methods to Characterize Synthesis and Degradation of Sphingomyelin at the Plasma Membrane and Its Impact on Lipid Raft Dynamics.- Association of Glycolipids and Growth Factor Receptors in Lipid Rafts.- Lipid Raft Isolation by Sucrose Gradient Centrifugation and Visualization of Raft-Located Proteins by Fluorescence Microscopy: The Use of Combined Techniques to Assess Fas/CD95 Location in Rafts during Apoptosis Triggering.- Elucidating the Role of Lipid Rafts on G Protein-Coupled Receptor Function in the Mouse Kidney: An In Vivo Approach.- Analysis of Lipids in Ceramide-Enriched Membrane Domains.- Chemotherapeutic Agents-Induced Ceramide Rich Platforms (CRPs) in Endothelial Cells and their Modulation.- Biophysical Analysis of Lipid Domains by Fluorescence Microscopy.- Biophysical Analysis of Lipid Domains in Mammalian and Yeast Membranes by Fluorescence Spectroscopy.- Characterization of Lipid Order and Domain Formation in Model Membranes Using Fluorescence Microscopy and Spectroscopy.- Characterization of the Effect of Sphingolipid Accumulation on Membrane Compactness, Dipole Potential, and Mobility of Membrane Components.- Super-Resolution Microscopy of Sphingolipids.- Detection of Functionalized Sphingolipid Analogues in Detergent Resistant Membranes of the Immune Cells.- Immunofluorescence Labeling of Lipid-Binding Proteins CERTs to Monitor Lipid Rafts Dynamics.- Cross-Link/Proximity Ligation Assay for Visualization of Lipid and Protein Complexes in Lipid Rafts.
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glycolipids;vesicle transport;Lipid-mediated affinity chromatography (LIMAC);neurodegenerative disease;mass spectrometry