Heterologous Protein Production in CHO Cells

Serum-Free and Protein-Free Media for the Cultivation of Recombinant Chinese Hamster Ovary Cells (CHO) Cell Lines.- Large-Scale Transient Transfection of Suspension-Adapted Chinese Hamster Ovary Cells for the Production of the Trimeric SARS-CoV-2 Spike Protein.- Splicing by Overlap Extension PCR for the Production of Fusion Proteins.- Codon and Signal Peptide Optimization to Enhance Therapeutic Antibody Production from CHO Cells.- Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells.- Conditional Knockdown of Endogenous MicroRNAs in CHO Cells Using TET-ON-SanDI Sponge Vectors.- MiRNA Chaining for Efficient Stable Overexpression to Improve Protein Quantity and Quality in CHO Cells.- Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells.- The 'Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity.- Recent Advancements in Proteomic Sample Preparation from Recombinant Chinese Hamster Ovary Cells.- Separation and Purification of CHO Secretome and Extracellular Vesicles for Proteome Analysis.- Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells.- LC-MS/MS Analysis to Study the Ubiquitin-Modified Proteome of Recombinant Chinese Hamster Ovary Cells.- Metabolomics of Chinese Hamster Ovary Cells.- Analytical Tools for HCP Detection, Identification, and Quantitation.- Quantitative High-Throughput and High-Sensitivity Glycoprofile Analysis of Therapeutic Glycoproteins Using HILIC-FLD and ESI-MS.

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• Biologia Celular (citologia)
• Doenças e desvios
• Bioquímica
• Terapia e terapêutica

Chinese hamster ovary cells;Biotherapeutics;Mammalian host cell line;Genetic cell manipulation;Recombinant protein products;Biopharmaceuticals