B-Cell Receptor Signaling

B-Cell Receptor Signaling

Methods and Protocols

Maity, Palash Chandra

Springer-Verlag New York Inc.

04/2025

300

Dura

9781071644416

Pré-lançamento - envio 15 a 20 dias após a sua edição

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Adoptive Transfer of B Cells In Vivo for Assessment of Their Immune Function.- Analysis of Germinal Center Reaction in Competitive Bone Marrow Chimeric Rag2-/-?c-/- Mice.- Immunophenotyping of B Cell Subpopulations by Flow Cytometry.- Evaluation of Constitutive or Induced Activation State of the VLA-4 Integrin in Human and Murine Samples.- Traction Force Microscopy for Studying B Lymphocyte Mechanosensing.- High-Resolution Imaging of Intracellular Trafficking of B Cell Receptor Using Specific Hybridization Internalization Probe (SHIP).- In-Depth, High-Throughput Analysis of BcR IG Clonality and Diversity Levels in Immune Repertoires.- Recombinant Production of B-Cell Receptor Fab Fragments from Chronic Lymphocytic Leukaemia in Mammalian Cells for Structural and Biochemical Studies.- Design, Production, and Optimization of Antigen-Specific Recombinant Antitumor Dimeric IgA Antibody.- Facilitating Gene Editing in Human Lymphoma Cells Using Murine Ecotropic ?-Retroviruses.- CRISPR/Cas9 Methods for Identification and Validation of Genes Regulating BCR-Mediated Antigen Uptake.- A Systems Biology Approach of Quantifying Signal Transduction to B Cell Proliferation and Differentiation.- Comparative Profiling of Regulatory Modules as a Tool for Identifying the Transcription Factor Network Linked to Leukemogenesis.- Studying Mitogen-Independent Proliferation in Murine B Cells.- B Cell Co-Culture in the Tumor Microenvironment of Solid Tumors Using Pancreatic Cancer as a Tumor Model.- A Comprehensive Methodology for Immortalizing Tumor-Infiltrating B Lymphocytes from Epithelial Cancers.- Quantification of Telomere Length in Peripheral Blood Mononuclear Cells Using Quantitative Polymerase Chain Reaction.- Generation and Reactivity Profiling of Functional Human Recombinant Monoclonal Antibodies.
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B-lymphocytes;bone marrow chimera model;Cell based assay;B cell receptor clustering;single cell technologies