Atherosclerosis
portes grátis
Atherosclerosis
Methods and Protocols
Ramji, Dipak
Springer-Verlag New York Inc.
03/2023
880
Mole
Inglês
9781071619261
15 a 20 dias
Descrição não disponível.
Atherosclerosis: Pathogenesis and key cellular processes, current and emerging therapies, key challenges and future research directions.- Key roles of inflammation in atherosclerosis: Mediators involved in orchestrating the inflammatory response and its resolution in the disease along with therapeutic avenues targeting inflammation.- Survey of in vitro model systems for investigation of key cellular processes associated with atherosclerosis.- Survey of approaches for investigation of atherosclerosis in vivo.- Exploring the associations between clonal hematopoiesis of indeterminate potential, myeloid malignancy and atherosclerosis.- Peptides as therapeutic agents for atherosclerosis.- Isolation and culturing of primary mouse and human macrophages.- Effective transfection and gene silencing of primary murine macrophages with small interfering RNA.- Monitoring celular proliferation, migration and apoptosis associated with atherosclerosis plaques invitro.- Monocyte adhesion assays for detecting endothelial cell activation in vascular inflammation and aterosclerosis.- Chick embryo ex vivo aortic sprouting assays for cardiovascular research.- Purification and analysis of circulating lipid particles.- Preparation of LDL, oxidation, methods of detection and applications in atherosclerosis research.- Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation.- A fluorescent based in vitro method to assess cholesterol efflux.- Monitoring the anisotropy and fluidity of the HDL monolayer as surrogates of HDL functionality.- Assessment of ex-vivo antioxidative potential of murine HDL in atherosclerosis.- Dual-fluorescence in vitro efferocytosis assay.- Investigation of mitochondrial bioenergetic profile and dysfunction in atherosclerosis.- Probing inflammasome activation in atherosclerosis.- Investigation of platelet aggregation in atherosclerosis.- Extracellular vesicles in atherosclerosis research.- The use of wire myography to investigate vascular tone and function.- Pig and mouse models of hyperlipidemia and atherosclerosis.- Use of rabbit models to study atherosclerosis.- Genetically engineered hamster models of dyslipidemia and atherosclerosis.- Atherosclerosis induced by adeno-associated virus encoding gain-of-function PCSK9.- Monitoring leukocyte migration during atherosclerosis in vivo.- Evaluation of plaque burden and lipid content in atherosclerotic plaques.- Monitoring cellularity and expression of key markers in atherosclerotic plaques.- Monitoring cellular proliferation and apoptosis in atherosclerosis plaques and intimal thickenings.- Investigation of atherosclerotic plaque vulnerability.- Use of mouse carotid artery ligation model of intimal thickening to probe vascular smooth muscle cell remodelling and function in atherosclerosis.- Assessing efferocytosis in atherosclerotic lesions in situ.- Monitoring atheroprotective macrophage cholesterol efflux in vivo.- Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis.- Whole mount preparation of mouse aorta for confocal microscopy studies of the intima.- Isolation of mouse aortic RNA for transcriptomics.- Redox proteomics analysis of atherosclerotic aortas: Application of the "OxICAT" method.- Intravital microscopy in atherosclerosis research.- Localization of long non-coding RNA in formalin-fixed-paraffin embedded vascular tissue using in-situ hybridization.- In vivo gene editing in lipid and atherosclerosis research.- Laser capture microdissection-based mRNA expression microarrays and single-cell RNA sequencing in atherosclerosis research.- Combined single cell RNA and single cell ?/? T cell receptor sequencing of the arterial wall in atherosclerosis.- Tissue clearing approaches in atherosclerosis.- Single cell antibody sequencing in atherosclerosis research.- Flow cytometry and mass cytometry for measuring the immune cell infiltrate in atherosclerotic arteries.- Contrast-enhanced ultrasound molecular imaging in atherosclerosis.- Nanoprobes for computed tomography and magnetic resonance imaging in atherosclerosis research.- Positron emission tomography in atherosclerosis research.- Three-dimensional visualization of atherosclerotic vessels by tissue clearing and light sheet fluorescence microscopy.- Intravascular fluorescence molecular imaging of atherosclerosis.
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inflammation;plaque rupture;inflammasome;endothelial cell proliferation;Pathogenesis
Atherosclerosis: Pathogenesis and key cellular processes, current and emerging therapies, key challenges and future research directions.- Key roles of inflammation in atherosclerosis: Mediators involved in orchestrating the inflammatory response and its resolution in the disease along with therapeutic avenues targeting inflammation.- Survey of in vitro model systems for investigation of key cellular processes associated with atherosclerosis.- Survey of approaches for investigation of atherosclerosis in vivo.- Exploring the associations between clonal hematopoiesis of indeterminate potential, myeloid malignancy and atherosclerosis.- Peptides as therapeutic agents for atherosclerosis.- Isolation and culturing of primary mouse and human macrophages.- Effective transfection and gene silencing of primary murine macrophages with small interfering RNA.- Monitoring celular proliferation, migration and apoptosis associated with atherosclerosis plaques invitro.- Monocyte adhesion assays for detecting endothelial cell activation in vascular inflammation and aterosclerosis.- Chick embryo ex vivo aortic sprouting assays for cardiovascular research.- Purification and analysis of circulating lipid particles.- Preparation of LDL, oxidation, methods of detection and applications in atherosclerosis research.- Monitoring modified lipoprotein uptake and macropinocytosis associated with macrophage foam cell formation.- A fluorescent based in vitro method to assess cholesterol efflux.- Monitoring the anisotropy and fluidity of the HDL monolayer as surrogates of HDL functionality.- Assessment of ex-vivo antioxidative potential of murine HDL in atherosclerosis.- Dual-fluorescence in vitro efferocytosis assay.- Investigation of mitochondrial bioenergetic profile and dysfunction in atherosclerosis.- Probing inflammasome activation in atherosclerosis.- Investigation of platelet aggregation in atherosclerosis.- Extracellular vesicles in atherosclerosis research.- The use of wire myography to investigate vascular tone and function.- Pig and mouse models of hyperlipidemia and atherosclerosis.- Use of rabbit models to study atherosclerosis.- Genetically engineered hamster models of dyslipidemia and atherosclerosis.- Atherosclerosis induced by adeno-associated virus encoding gain-of-function PCSK9.- Monitoring leukocyte migration during atherosclerosis in vivo.- Evaluation of plaque burden and lipid content in atherosclerotic plaques.- Monitoring cellularity and expression of key markers in atherosclerotic plaques.- Monitoring cellular proliferation and apoptosis in atherosclerosis plaques and intimal thickenings.- Investigation of atherosclerotic plaque vulnerability.- Use of mouse carotid artery ligation model of intimal thickening to probe vascular smooth muscle cell remodelling and function in atherosclerosis.- Assessing efferocytosis in atherosclerotic lesions in situ.- Monitoring atheroprotective macrophage cholesterol efflux in vivo.- Flow cytometry analysis of hematopoietic stem/progenitor cells and mature blood cell subsets in atherosclerosis.- Whole mount preparation of mouse aorta for confocal microscopy studies of the intima.- Isolation of mouse aortic RNA for transcriptomics.- Redox proteomics analysis of atherosclerotic aortas: Application of the "OxICAT" method.- Intravital microscopy in atherosclerosis research.- Localization of long non-coding RNA in formalin-fixed-paraffin embedded vascular tissue using in-situ hybridization.- In vivo gene editing in lipid and atherosclerosis research.- Laser capture microdissection-based mRNA expression microarrays and single-cell RNA sequencing in atherosclerosis research.- Combined single cell RNA and single cell ?/? T cell receptor sequencing of the arterial wall in atherosclerosis.- Tissue clearing approaches in atherosclerosis.- Single cell antibody sequencing in atherosclerosis research.- Flow cytometry and mass cytometry for measuring the immune cell infiltrate in atherosclerotic arteries.- Contrast-enhanced ultrasound molecular imaging in atherosclerosis.- Nanoprobes for computed tomography and magnetic resonance imaging in atherosclerosis research.- Positron emission tomography in atherosclerosis research.- Three-dimensional visualization of atherosclerotic vessels by tissue clearing and light sheet fluorescence microscopy.- Intravascular fluorescence molecular imaging of atherosclerosis.
Este título pertence ao(s) assunto(s) indicados(s). Para ver outros títulos clique no assunto desejado.
